General Cell Lines and Cell Cultures
Cell Applications Inc Bovine Pulmonary Artery Endothelial Cells Total Kit
Bovine Pulmonary Artery Endothelial Cells Total Kit:(B302-05) Cryopreserved (BPAEC), 1 vial(B211-500) Bovine Endothelial Cells Growth Media, 500ml(090K) Subculture Reagent Kit
Biochain Institute Inc Frozen Tissue Array - 37 Different Colon Tumors and 3 Corresponding Normal Controls, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's human tumor frozen tissue arrays are designed for studying tumor tissue specific cellular localization of genes or proteins of interest in different human tissues. Same kind of tumors and corresponding normal tissues are mounted on positively charged slides.
Eurofins Discoverx PathHunter OXER1 Total Internalization U2OS Cell Line, 2 vials. (93-0955C3)
The PathHunter OXER1 Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of OXER1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged OXER1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the OXER1 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Cayman Chemical OrexIn B amIdehumntrIfl 500ug
A hypothalamic neuropeptide; an OX receptor agonist (IC50s = 420 and 36 nM, respectively, for human OX1 and OX2 receptors); induces transient increases in intracellular calcium in CHO cells stably expressing OX1 and OX2 (EC50s = 2,500 and 60 nM, respectively) and in primary rat VTA dopaminergic neurons; stimulates arachidonic acid release from CHO cells expressing human recombinant OX1 (EC50 = 4.17 nM); stimulates food intake in freely feeding mice (30 nmol, i.c.v.); stimulates food intake in goldfish (10 ng/g, i.c.v.)
Biochain Institute Inc Paraffin Tissue Section Panel - Human Adult Normal Tissue, Brain II, 7 different tissues, 5 slides/PK
BioChain's human adult normal multiple tissue panels are designed for studying tissue specific cellular localization of genes or proteins of interest in different human normal tissues. Tissues mounted on positively charged glass slides are organized either by organs (major organ multiple tissue panels), or by integrated major systems (organ systems multiple tissue panels). Documentation of tissues' clinical histories is available upon request. Identical format total RNA Northern Blots are available, and cDNA from those tissues may also be available for PCR detection.
Eurofins Discoverx PathHunter DLD1 FFAR1 β-Arrestin Cell Line, 2 vials. (93-0950C13)
The PathHunter FFAR1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. free fatty acids) based activation of FFAR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged FFAR1 and EA-tagged β-Arrestin-2. Activation of FFAR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Amsbio LLC Cryopreserved| Subcutaneous Preadipocytes| FACE (1 million cells/vial).
Cryopreserved Subcutaneous Preadipocytes FACE (1 million cells/vial).
Eurofins Discoverx PathHunter U2OS CD137 Signaling Assay, 2 vials. (93-1089C3)
The PathHunter CD137 Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of CD137 induced non-canonical NF-κB signaling via detection of NIK stabilization. This product uses EFC technology. The included cell line overexpresses ED-tagged NIK (NF-kappa-B Inducing Kinase) protein as well as untagged CD137. Upon activation of the receptor by its ligand or an antibody, NIK is stabilized, allowing detection of EFC signal when treated with exogenous EA. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/checkpoint.
Eurofins Discoverx PathHunter U2OS HTR5A β-Arrestin Cell Line, 2 vials. (93-0700C3)
The PathHunter HTR5A cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin) based activation of HTR5A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR5A and EA-tagged β-Arrestin-2. Activation of HTR5A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx Ready-to-Assay S1P4 Lysophospholipid Receptor Frozen Cells, 2 Vials of Cells. (HTS192RTA)
The Ready-to-Assay S1P4 Lysophospholipid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. S1P) based activation of S1PR4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses S1PR4 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of S1PR4, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx PathHunter CHO-K1 GPR18 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0560C2A)
The PathHunter GPR18 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR18 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR18 and EA-tagged β-Arrestin-2. Activation of GPR18 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Quantitative Genomic; RNA from Human; coronavirus 229E
Quantitative genomic RNA from Human coronavirus 229E (ATCC VR-740) can be used for assay development, verification, and validation as well as monitoring of day to day test variation and lot to lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.
ATCC HMCB; Melanoma; Human (Homo sapiens)
HMCB [ Human Melanoma Cell Bowes] is an epithelial cell line that was isolated from the skin of a donor with melanoma.
Biochain Institute Inc Paraffin Tissue Section - Human Kidney Tumor: Renal Cell Carcinoma, 5 sides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from a single human tumor. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Eurofins Discoverx Ready-to-Assay IP1 Prostanoid Receptor Frozen Cells, 2 Vials of Cells. (HTS131LRTA)
The Ready-to-Assay IP1 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin I2) based activation of PTGIR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGIR alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGIR, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.